MOLECULAR GENETIC DISORDERS IN THE VHL GENE AND METHYLATION OF SOME SUPPRESSOR GENES IN SPORADIC CLEAR-CELL RENAL CARCINOMAS

D. S. Mikhailenko; Михайленко Д. С.; M. V. Grigoryeva; Григорьева М. В.; V. V. Zemlyakova; Землякова В. В.; V. V. Shkarupo; Шкарупо В. В.; E. S. Yakovleva; Яковлева Е. С.; D. A. Nosov; Носов Д. А.; L. N. Lyubchenko; Любченко Л. Н.; S. A. Tyulyandin; Тюляндин С. А.; R. V. Kurynin; Курынин Р. В.; A. M. Popov; Попов А. М.; D. V. Zaletayev; Залетаев Д. В.; I. G. Rusakov; Русаков И. Г.

doi:10.17650/1726-9776-2010-6-2-32-37

MOLECULAR GENETIC DISORDERS IN THE VHL GENE AND METHYLATION OF SOME SUPPRESSOR GENES IN SPORADIC CLEAR-CELL RENAL CARCINOMAS

Authors: Mikhailenko D.S.¹, Grigoryeva M.V.², Zemlyakova V.V.¹, Shkarupo V.V.¹, Yakovleva E.S.³, Nosov D.A.³, Lyubchenko L.N.³, Tyulyandin S.A.³, Kurynin R.V.⁴, Popov A.M.⁵, Zaletayev D.V.², Rusakov I.G.²
Affiliations:
1. Medical Genetic Research Center, Russian Academy of Medical Sciences
2. P.A. Herzen Moscow Research Oncological Institute, Russian Agency for Health Care
3. N.N. Blokhin Russian Cancer Research Center, Russian Academy of Medical Sciences, Moscow
4. Urology Clinic, I.M. Sechenov Moscow Medical Academy, Russian Academy of Medical Sciences
5. Medical Radiology Research Center, Russian Academy of Medical Sciences, Obninsk
Issue: Vol 6, No 2 (2010)
Pages: 32-37
Section: DIAGNOSIS AND TREATMENT OF URINARY SYSTEM TUMORS. RENAL CANCER
Published: 30.06.2010
URL: https://oncourology.abvpress.ru/oncur/article/view/50
DOI: https://doi.org/10.17650/1726-9776-2010-6-2-32-37
ID: 50

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Abstract

Renal carcinoma (RC) is one of ten most common malignancies in adults and an urgent problem of modern oncology. The purpose of the study was to make a molecular genetic analysis of a number of suppressor genes in RC, which was aimed at searching for and characterizing the potential markers of the disease. Two hundred and nine RC samples were examined, of them there were 192 clear-cell carcinomas. VHL gene mutations were detected by single-strand conformation polymorphism and sequence analyses while the methylation of suppressor genes was by the methylation-sensitive polymerase chain reaction. Somatic VHL mutations were determined in 35.4% of cases of clear-cell RC (CCRC). VHL gene disorders were found in 53.7% of patients with Stage 1, which counts in favor of VHL inactivation in early-stage CCRC. The methylation of the VHL, RASSF1, FHIT, and CDH1 genes was identified in 12, 56, 58.4, and 46.4% of primary tumors, respectively; that of at least one gene was in 84.1% of the samples. The hypermethylation of the RASSF1 gene was associated with late stages (p = 0.015) and the presence of metastases (p = 0.036); that of the CDH1 gene was related to the progression, invasion, and dissemination of primary tumors (p = 0.009, 0.039, and 0.002, respectively). The findings show it possible to use an analysis of abnormalities in the VHL gene and the methylation of the RASSF1 and CDH1 genes to develop a system of molecular genetic markers of RC.

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clear-cell renal carcinoma, mutation, molecular genetic analysis, suppressor genes, methylation, sequencing

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MOLECULAR GENETIC DISORDERS IN THE VHL GENE AND METHYLATION OF SOME SUPPRESSOR GENES IN SPORADIC CLEAR-CELL RENAL CARCINOMAS

Full Text

Abstract

Keywords

About the authors

References

Supplementary files