Search for new microRNAs for differential diagnosis of teratomas

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Abstract

Aim. Search for new microRNAs as markers for differential diagnosis of teratoma and live tumor tissues.

Materials and methods. In the study, freshly frozen tissue samples obtained after orchifuniculectomy (n = 46) and retroperitoneal lymph node dissection (n = 42) in patients with TGCT diagnosis were analyzed. MicroRNAs were isolated from tissue samples and analyzed using reverse transcription polymerase chain reaction.

Results. After studying the Cancer Genome Atlas, microRNA-196a-5p and microRNA-200b-3p with differential expression in tissue samples of different histological types of TGCT were identified. These microRNA are significantly overexpressed in non-seminomas, and their expression levels significantly differ from expression levels in normal tissues and seminomas. The level of microRNA-196b-5p expression in teratomas obtained after retroperitoneal lymph node dissection is significantly higher than in seminomas, and microRNA-200b-3p expression level is significantly higher than in tissues with necrotic changes. ROC analysis has shown 89 % sensitivity and 79 % specificity for microRNA-196b-5p as a diagnostic marker. For microRNA-200b-3p, sensitivity and specificity were 75 and 89 %, respectively.

Conclusion. In this study, we have shown for the first time that microRNA-196a-5p and microRNA-200b-3p have high diagnostic potential as markers for identification of various types of TGCTs including teratoma.

About the authors

K. S. Nemtseva

N.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of Russia

Author for correspondence.
Email: karoline11111@mail.ru
ORCID iD: 0000-0002-1002-161X

Karolina Stanislavovna Nemtseva

 24 Kashirskoe Shosse, Moscow 115522

Russian Federation

V. B. Matveev

N.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of Russia

ORCID iD: 0000-0001-7748-9527

 24 Kashirskoe Shosse, Moscow 115522

Russian Federation

N. V. Elkina

N.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of Russia

ORCID iD: 0000-0002-0503-6016

 24 Kashirskoe Shosse, Moscow 115522

Russian Federation

M. D. Fedorova

N.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of Russia

ORCID iD: 0000-0002-8813-7516

 24 Kashirskoe Shosse, Moscow 115522

Russian Federation

D. S. Elkin

N.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of Russia

ORCID iD: 0000-0002-4793-6063

 24 Kashirskoe Shosse, Moscow 115522

Russian Federation

O. A. Khalmurzaev

N.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of Russia

ORCID iD: 0000-0001-7500-1815

 24 Kashirskoe Shosse, Moscow 115522

Russian Federation

M. I. Beyshembaev

National Center for Oncology and Hematology, Ministry of Health of the Kyrgyz Republic

ORCID iD: 0000-0002-3396-4047

92 Akhunbayev St., Bishkek 720020

Kyrgyzstan

G. A. Arakelyan

N.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of Russia

ORCID iD: 0000-0003-3528-1466

 24 Kashirskoe Shosse, Moscow 115522

Russian Federation

S. V. Vinokurova

N.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of Russia

ORCID iD: 0000-0003-1615-3928

 24 Kashirskoe Shosse, Moscow 115522

Russian Federation

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