The first experience of using prostate cancer organoids as a model for personalized selection of drugs
https://doi.org/10.17650/1726-9776-2023-19-2-41-46
Abstract
Background. A promising experimental approach to the personalized selection of treatment regimens is the study of the sensitivity of tumor cells to drugs in vitro on tumor organoids.
Aim. To generate a culture of prostate tumor organoids and to assess the effectiveness of the chemotherapeutic drug docetaxel used to treat prostate cancer on this culture.
Materials and methods. The initial tissue was dissociated using gentleMACS Octo homogenizer. Next, the cells were cultured in matrix Matrigel with addition of a serum-free complete nutrient medium. For histological analysis, organoids were fixed in a 10 % formalin solution, followed by staining with hematoxylin and eosin according to the standard protocol. Cell viability was assessed using MTS assay.
Results. In this work, we generated a new culture of prostate cancer cells. The histological analysis confirmed that the resulting organoids consist of tumor epithelial cells. As a result of the cytotoxic test, it was shown that in this case docetaxel (82.9 %; p = 0.32) didn’t reduce statistically significantly the viability of prostate cancer cells compared to the control.
Conclusion. The use of tumor organoids of prostate cancer for selection of an optimal treatment regimen is a promising experimental technology, however, further research is necessary for its introduction into practice.
Supporting agencies: The study was performed with the support of Russian Science Foundation (Project No. 19-15-00397).
About the Authors
S. V. Nikulin
https://www.hse.ru/org/persons/357576526
National Medical Research Radiological Center, Ministry of Health of Russia; National Research University “Higher School of Economics”
Russian Federation
Competing Interests:
National Medical Research Radiological Center, Ministry of Health of Russia; National Research University “Higher School of Economics”
Russian Federation
Sergey Vyacheslavovich Nikulin
3 2nd Botkinskiy Proezd, Moscow 125284, Russia
20 Myasnitskaya St., Moscow 101000, Russia
Competing Interests:
нет
B. Ya. Alekseev
National Medical Research Radiological Center, Ministry of Health of Russia;
Medical Institute of Continuing Education, Russian Biotechnological University
Russian Federation
Competing Interests:
Russian Federation
3 2nd Botkinskiy Proezd, Moscow 125284, Russia
11 Volokolamskoe Shosse, Moscow 125080, Russiа
Competing Interests:
нет
A. A. Poloznikov
National Medical Research Radiological Center, Ministry of Health of Russia;
National Research University “Higher School of Economics”
Russian Federation
Competing Interests:
Russian Federation
3 2nd Botkinskiy Proezd, Moscow 125284, Russia
20 Myasnitskaya St., Moscow 101000, Russia
Competing Interests:
нет
A. I. Osipyants
National Medical Research Radiological Center, Ministry of Health of Russia;
Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology, Ministry of Health of Russia
Russian Federation
Competing Interests:
Russian Federation
3 2nd Botkinskiy Proezd, Moscow 125284, Russia
1 Samory Mashela St., Moscow 117997, Russia
Competing Interests:
нет
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For citations:
Nikulin S.V., Alekseev B.Ya., Poloznikov A.A., Osipyants A.I. The first experience of using prostate cancer organoids as a model for personalized selection of drugs. Cancer Urology. 2023;19(2):41-46. (In Russ.) https://doi.org/10.17650/1726-9776-2023-19-2-41-46
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