Изотермическая петлевая амплификация: эффективный метод экспресс-диагностики в онкологии
Аннотация
В обзоре обсуждается новый метод молекулярной биологии - изотермическая петлевая амплификация (LAMP), приобретающий в последнее время все большую популярность, особенно для целей диагностики. В отличие от ПЦР, амплификация ДНК или РНК происходит при постоянной температуре (около 650С) с использованием четырех или шести праймеров. Метод отличается высокой специфичностью, чувствительностью и быстротой. Для него не нужны дорогостоящие приборы, а детекция продуктов реакции может быть проведена невооруженным глазом. LAMP успешно применен для диагностики десятков вирусов, бактерий и других патогенов, в том числе в составе пищевых продуктов, а также для детекции однонуклеотидных полиморфизмов. Недавно была создана модификация LAMP, предназначенная для детекции метастазов. Она получила название OSNA (one-step nucleic acid amplification). Оказалось, что OSNA обладает практически такой же чувствительностью и специфичностью, как и стандартные диагностические методы, а иногда даже превосходит их. Особое внимание уделено механизму LAMP, дизайну праймеров и диагностике онкологических заболеваний с помощью OSNA.
Об авторах
Ю. А. Макарова
ФГБУ «Национальный медицинский исследовательский центр радиологии» Минздрава России;
ФГБУН «Институт молекулярной биологии им. В.А. Энгельгардта Российской академии наук»
Россия
249031 Обнинск, ул. Королева, 4; 119991 Москва, ул. Вавилова, 32
Россия
249031 Обнинск, ул. Королева, 4; 119991 Москва, ул. Вавилова, 32
А. А. Зотиков
ФГБУ «Национальный медицинский исследовательский центр радиологии» Минздрава России
Россия
249031 Обнинск, ул. Королева, 4
Россия
249031 Обнинск, ул. Королева, 4
Г. А. Белякова
ФГАОУ ВО Первый Московский государственный медицинский университет им. И.М. Сеченова Минздрава России
Россия
119991 Москва, ул. Трубецкая, 8, стр. 2
Россия
119991 Москва, ул. Трубецкая, 8, стр. 2
Б. Я Алексеев
ФГБУ «Национальный медицинский исследовательский центр радиологии» Минздрава России
249031 Обнинск, ул. Королева, 4
249031 Обнинск, ул. Королева, 4
М. Ю. Шкурников
ФГАОУ ВО Первый Московский государственный медицинский университет им. И.М. Сеченова Минздрава России
Россия
119991 Москва, ул. Трубецкая, 8, стр. 2
Россия
119991 Москва, ул. Трубецкая, 8, стр. 2
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При поддержке: Российский научный фонд (проект № 16-15-00290).
Для цитирования:
Макарова Ю.А., Зотиков А.А., Белякова Г.А., Алексеев Б.Я., Шкурников М.Ю. Изотермическая петлевая амплификация: эффективный метод экспресс-диагностики в онкологии. Онкоурология. 2018;14(2):88-99. https://doi.org/10.17650/1726-9776-2018-14-2-88-99
For citation:
Makarova Y.A., Zotikov A.A., Belyakova G.A., Alekseev B.Y., Shkurnikov M.Y. Loop-mediated isothermal amplification: an effective method for express-diagnostics of cancer. Cancer Urology. 2018;14(2):88-99. (In Russ.) https://doi.org/10.17650/1726-9776-2018-14-2-88-99
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