Comparative analysis of the PCA3 gene expression in sediments and exosomes isolated from urine

D. S. Mikhaylenko; Михайленко Д. С.; A. A. Novikov; Новиков А. А.; M. V. Grigor’eva; Григорьева М. В.; G. D. Efremov; Ефремов Г. Д.; A. V. Sivkov; Сивков А. В.; N. Yu. Safronova; Сафронова Н. Ю.; K. S. Sorokin; Сорокин К. С.; M. Yu. Zemskova; Земскова М. Ю.; M. V. Nemtsova; Немцова М. В.; B. Ya. Alekseev; Алексеев Б. Я.; A. D. Kaprin; Каприн А. Д.

doi:10.17650/1726-9776-2017-13-3-54-60

Comparative analysis of the PCA3 gene expression in sediments and exosomes isolated from urine

Authors: Mikhaylenko D.S.¹, Novikov A.A.², Grigor’eva M.V.², Efremov G.D.², Sivkov A.V.², Safronova N.Y.², Sorokin K.S.³, Zemskova M.Y.⁴, Nemtsova M.V.⁵, Alekseev B.Y.², Kaprin A.D.²
Affiliations:
1. N.A. Lopatkin Research Institute of Urology and Interventional Radiology – branch of the National Medical Research Radiological Center, Ministry of Health of Russia Institute of Molecular Medicine, I. M. Sechenov First Moscow State Medical University, Ministry of Health of Russia Research Center for Medical Genetics
2. N.A. Lopatkin Research Institute of Urology and Interventional Radiology – branch of the National Medical Research Radiological Center, Ministry of Health of Russia
3. Prostagnost Company
4. Prostagnost Company G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms
5. Institute of Molecular Medicine, I. M. Sechenov First Moscow State Medical University, Ministry of Health of Russia
Issue: Vol 13, No 3 (2017)
Pages: 54-60
Section: PROSTATE CANCER
Published: 30.09.2017
URL: https://oncourology.abvpress.ru/oncur/article/view/685
DOI: https://doi.org/10.17650/1726-9776-2017-13-3-54-60
ID: 685

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Abstract

Introduction. Prostate cancer (PCa) is one of the common oncological diseases in men. Expression of the PCA3 gene in urine is currently used as a molecular genetic marker of PCa.

Objective: to comparative analysis of the PCA3 expression in urine sediments and exosomes for the determination of the biomaterial, which allows detecting the PCA3 expression in more efficient manner.

Materials and methods. The 12 patients with different stages of PCa and 8 control samples were examined.

Results. The diagnostic accuracy of the PCA3 gene expression analysis in this cohort exceeded 90 %. We had not obtained significant differences in the sensitivity and specificity of the PCA3 hyperexpression in the urine sediments compared with exosomes. This result indicates in favor to using urine sediment for the PCA3 analysis as a biomaterial with less time-consuming sample preparation, although the possible advantage of exosomes for the analysis of the expression marker panels requires further studies.

Keywords

PCA3, gene expression, exosome, non-invasive diagnostics

About the authors

D. S. Mikhaylenko

N.A. Lopatkin Research Institute of Urology and Interventional Radiology – branch of the National Medical Research Radiological Center, Ministry of Health of Russia

Institute of Molecular Medicine, I. M. Sechenov First Moscow State Medical University, Ministry of Health of Russia

Research Center for Medical Genetics

Author for correspondence.
Email: dimserg@mail.ru
ORCID iD: 0000-0001-9780-8708

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