Cancer UrologyCancer UrologyОнкоурология1726-97761996-1812Publishing House ABV Press67610.17650/1726-9776-2017-13-2-63-66DIAGNOSIS AND TREATMENT OF URINARY SYSTEM TUMORS. PROSTATE CANCERДИАГНОСТИКА И ЛЕЧЕНИЕ ОПУХОЛЕЙ МОЧЕПОЛОВОЙ СИСТЕМЫ. Рак предстательной железыApplication of loop-mediated isothermal amplification of DNA for diagnosis of prostate cancer micrometastases in the lymph nodesПрименение петлевой изотермической амплификации ДНК для диагностики микрометастазов рака предстательной железы в лимфатические узлыShkurnikovM. Yu.ШкурниковМ. Ю.
Russian Federation

3 2nd Botkinskiy Proezd, Moscow 125284

Шкурников Максим Юрьевич - к.м.н., старший научный сотрудник отдела трансляционной онкологии 

SPIN-код: 7023-4575 

125284 Москва, 2-й Боткинский проезд, 3 

mshkurnikov@gmail.comZotikovA. A.ЗотиковА. А.
Russian Federation

Зотиков Александр Александрович

azdoc90@gmail.comBelyakovM. M.БеляковМ. М.
Russian Federation

Беляков Михаил Михайлович

mnioi_morphology@mail.ruNushkoK. M.НюшкоК. М.
Russian Federation

Нюшко Кирилл Михайлович

kirandja@yandex.ruFomichevaK. A.ФомичеваК. А.
Russian Federation

Фомичева Карина Алексеевна

karina-fomicheva@yandex.ruKnyazevE. N.КнязевЕ. Н.
Russian Federation

Князев Евгений Николаевич

knyazevevg@gmail.comSanevichM. R.СаневичМ. Р.
Russian Federation

Саневич Марк Рафаилович

marksanevich@gmail.comIvanovichV. V.ВечоркоВ. И.
Russian Federation

Вечорко Валерий Иванович

vvechorko@yandex.ruAlekseevB. Ya.АлексеевБ. Я.
Russian Federation

Алексеев Борис Яковлевич

byalekseev@mail.ruP.A. Hertzen Moscow Oncology Research Institute – branch of the National Medical Research Radiological Center, Ministry of Health of RussiaМосковский научно-исследовательский онкологический институт им. П.А. Герцена – филиал ФГБУ «Национальный медицинский исследовательский радиологический центр» Минздрава России3006201713263662004201710052017https://oncourology.abvpress.ru/oncur/article/view/676

A comparative analysis of the results of diagnosis of cancer metastases in the lymph nodes using a method of one-step nucleic acids amplification (OSNA) and morphological methods was performed. Comparable sensitivity and specificity of OSNA and histological examination of a lymph node were observed. 

Проведен сравнительный анализ результатов выявления метастазов рака в лимфатических узлах с помощью OSNA и морфологическими методами. Показана сопоставимая чувствительность и специфичность OSNA и гистологического исследования лимфатического узла.

prostate cancermicrometastasispolymerase chain reactionOSNALAMPрак предстательной железымикрометастазПЦРOSNALAMPРоссийский научный фонд1. Состояние онкологической помощи населению России в 2015 году. Под ред. А.Д. Каприна, В.В. Старинского, Г.В. Петровой. М.: МНИОИ им. П.А. Герцена – филиал ФГБУ «НМИРЦ» Минздрава России, 2016. 236 с. [State of oncological care in Russia in 2015. Eds.: A.D. Kaprin, V.V. Starinskiy, G.V. Petrova. Moscow: MNIOI im. P.A. Gertsena – filial FGBU “NMIRTS” Minzdrava Rossii, 2016. 236 p. (In Russ.)].Состояние онкологической помощи населению России в 2015 году. Под ред. А.Д. Каприна, В.В. Старинского, Г.В. Петровой. М.: МНИОИ им. П.А. Герцена – филиал ФГБУ «НМИРЦ» Минздрава России, 2016. 236 с. [State of oncological care in Russia in 2015. Eds.: A.D. Kaprin, V.V. Starinskiy, G.V. Petrova. Moscow: MNIOI im. P.A. Gertsena – filial FGBU “NMIRTS” Minzdrava Rossii, 2016. 236 p. (In Russ.)].2. Mottet N., Bellmunt J., Bolla M. et al. EAU-ESTRO-SIOG guidelines on prostate cancer. Part 1: screening, diagnosis, and local treatment with curative intent. Eur Urol 2017;4(71):618–29. DOI: 10.1016/j.eururo.2016.08.003. PMID: 27568654.Mottet N., Bellmunt J., Bolla M. et al. EAU-ESTRO-SIOG guidelines on prostate cancer. Part 1: screening, diagnosis, and local treatment with curative intent. Eur Urol 2017;4(71):618–29. DOI: 10.1016/j.eururo.2016.08.003. PMID: 27568654.3. Leyh-Bannurah S.R., Budäus L., Pompe R. et al. North American population-based validation of the National comprehensive cancer network practice Guideline recommendation of pelvic lymphadenectomy in contemporary prostate cancer. Prostate 2017;77(5):542–8. DOI: 10.1002/pros.23292. PMID: 28093788.Leyh-Bannurah S.R., Budäus L., Pompe R. et al. North American population-based validation of the National comprehensive cancer network practice Guideline recommendation of pelvic lymphadenectomy in contemporary prostate cancer. Prostate 2017;77(5):542–8. DOI: 10.1002/pros.23292. PMID: 28093788.4. Williams S.K., Rabbani F. Complications of lymphadenectomy in urologic surgery. Urol Clin North Am 2011;38(4):507–18. DOI: 10.1016/j.ucl.2011.07.013. PMID: 22045181.Williams S.K., Rabbani F. Complications of lymphadenectomy in urologic surgery. Urol Clin North Am 2011;38(4):507–18. DOI: 10.1016/j.ucl.2011.07.013. PMID: 22045181.5. Schilling D., Hennenlotter J., Gakis G. et al. Prospective assessment of histological serial sectioning of pelvic lymph nodes in prostate cancer: a cost-benefit analysis. BJU Int 2012;110(6 Pt B): 166–71. DOI: 10.1111/j.1464-410X.2012.10928.x. PMID: 22314026.Schilling D., Hennenlotter J., Gakis G. et al. Prospective assessment of histological serial sectioning of pelvic lymph nodes in prostate cancer: a cost-benefit analysis. BJU Int 2012;110(6 Pt B): 166–71. DOI: 10.1111/j.1464-410X.2012.10928.x. PMID: 22314026.6. Karalak A., Homcha-Em P. Occult axillary lymph node metastases discovered by serial section in node-negative breast cancer. J Med Assoc Thai 1999;82(10):1017–9. PMID: 10561965.Karalak A., Homcha-Em P. Occult axillary lymph node metastases discovered by serial section in node-negative breast cancer. J Med Assoc Thai 1999;82(10):1017–9. PMID: 10561965.7. Samatov T.R., Shkurnikov M.U., Tonevitskaya S.A., Tonevitsky A.G. Modelling the metastatic cascade by in vitro microfluidic platforms. Prog Histochem Cytochem 2015;49(4):21–9. DOI: 10.1016/j.proghi.2015.01.001. PMID: 25759320.Samatov T.R., Shkurnikov M.U., Tonevitskaya S.A., Tonevitsky A.G. Modelling the metastatic cascade by in vitro microfluidic platforms. Prog Histochem Cytochem 2015;49(4):21–9. DOI: 10.1016/j.proghi.2015.01.001. PMID: 25759320.8. Tsujimoto M., Nakabayashi K., Yoshidome K. et al. One-step nucleic acid amplification for intraoperative detection of lymph node metastasis in breast cancer patients. Clin Cancer Res 2007;13(16):4807–16. DOI: 10.1158/1078-0432.CCR-06-2512. PMID: 17699859.Tsujimoto M., Nakabayashi K., Yoshidome K. et al. One-step nucleic acid amplification for intraoperative detection of lymph node metastasis in breast cancer patients. Clin Cancer Res 2007;13(16):4807–16. DOI: 10.1158/1078-0432.CCR-06-2512. PMID: 17699859.9. Notomi T., Okayama H., Masubuchi H. et al. Loop-mediated isothermal amplification of DNA. Nucleic Acids Res 2000;28(12):E63. PMID: 10871386.Notomi T., Okayama H., Masubuchi H. et al. Loop-mediated isothermal amplification of DNA. Nucleic Acids Res 2000;28(12):E63. PMID: 10871386.10. Nagamine K., Hase T., Notomi T. Accelerated reaction by loop-mediated isothermal amplification using loop primers. Mol Cell Probes 2002;16(3):223–9. PMID: 12144774.Nagamine K., Hase T., Notomi T. Accelerated reaction by loop-mediated isothermal amplification using loop primers. Mol Cell Probes 2002;16(3):223–9. PMID: 12144774.11. Yaguchi Y., Sugasawa H., Tsujimoto H. et al. One-step nucleic acid amplification (OSNA) for the application of sentinel node concept in gastric cancer. Ann Surg Oncol 2011;18(8): 2289–96. DOI: 10.1245/s10434-011-1591-9. PMID: 21301968.Yaguchi Y., Sugasawa H., Tsujimoto H. et al. One-step nucleic acid amplification (OSNA) for the application of sentinel node concept in gastric cancer. Ann Surg Oncol 2011;18(8): 2289–96. DOI: 10.1245/s10434-011-1591-9. PMID: 21301968.12. Inoue M., Hiyama K., Nakabayashi K. et al. An accurate and rapid detection of lymph node metastasis in non-small cell lung cancer patients based on one-step nucleic acid amplification assay. Lung Cancer 2012;78(3):212–8. DOI: 10.1016/j.lungcan.2012.08.018. PMID: 23026640.Inoue M., Hiyama K., Nakabayashi K. et al. An accurate and rapid detection of lymph node metastasis in non-small cell lung cancer patients based on one-step nucleic acid amplification assay. Lung Cancer 2012;78(3):212–8. DOI: 10.1016/j.lungcan.2012.08.018. PMID: 23026640.13. Kaczka K., Fendler W., Borowiec M. et al. One-step nucleic acid amplification testing in medullary thyroid cancer lymph nodes: a case series. Arch Med Sci 2015;1(11):137–41. DOI: 10.5114/aoms.2015.49206. PMID: 25861300.Kaczka K., Fendler W., Borowiec M. et al. One-step nucleic acid amplification testing in medullary thyroid cancer lymph nodes: a case series. Arch Med Sci 2015;1(11):137–41. DOI: 10.5114/aoms.2015.49206. PMID: 25861300.14. Tamaki Y. One-step nucleic acid amplification (OSNA): where do we go with it? Int J Clin Oncol 2017;22(1):3–10. DOI: 10.1007/s10147-016-1030-9. PMID: 27549784Tamaki Y. One-step nucleic acid amplification (OSNA): where do we go with it? Int J Clin Oncol 2017;22(1):3–10. DOI: 10.1007/s10147-016-1030-9. PMID: 27549784