Cancer UrologyCancer UrologyОнкоурология1726-97761996-1812Publishing House ABV Press18910.17650/1726-9776-2010-6-3-12-15DIAGNOSIS AND TREATMENT OF URINARY SYSTEM TUMORS. RENAL CANCERДИАГНОСТИКА И ЛЕЧЕНИЕ ОПУХОЛЕЙ МОЧЕПОЛОВОЙ СИСТЕМЫ. Рак почкиTHE ACTIVITY OF PROTEASOMES AND THEIR SUBUNIT COMPOSITION IN CANCER OF THE KIDNEY AND URINARY BLADDERАКТИВНОСТЬ ПРОТЕАСОМ И ИХ СУБЪЕДИНИЧНЫЙ СОСТАВ ПРИ РАКЕ ПОЧКИ И МОЧЕВОГО ПУЗЫРЯSpirinaL. V.СпиринаЛ. В.
Russian Federation
gusi70@list.ruKondakovaI. V.КондаковаИ. В.
Russian Federation
gusi70@list.ruUsyninE. A.УсынинЕ. А.
Russian Federation
gusi70@list.ruSharovaN. P.ШароваН. П.
Russian Federation
gusi70@list.ruCancer Research Institute, Siberian Branch, Russian Academy of Medical Sciences, TomskНИИ онкологии СО РАМН, ТомскN.K. Koltsov Institute of Developmental Biology, Russian Academy of Sciences, MoscowИнститут биологии развития им. Н.К. Кольцова РАН, Москва300920106312153107201431072014https://oncourology.abvpress.ru/oncur/article/view/189

The study revealed a reduction in the chemotrypsin-like activity of the total pool of proteasomes and their 26S and 20S pools in the tissue of clear-cell carcinoma of the kidney versus intact tissue. Renal cancer tissue was found to contain the higher levels of the proteasome subunits α1α2α3α5α6α7 than did intact tissues, which was accompanied by the lower content of the subunits LMP2 and PA28β. A urinary bladder tumor showed the enhanced activity of the 26S proteasomes and the lower expression of α1α2α3α5α6α7 and the elevated level of the LMP2 subunit as part of proteasomes. Intact renal tissue 26S proteasome activity was found to be related to late tumor spread. Urinary bladder cancer tissue showed a correlation between the expression of the proteasome subunits α1α2α3α5α6α7 and the size of a tumor and that between 26 proteasome activity and the degree of histological tumor differentiation.

В результате проведенного исследования выявлено снижение химотрипсиноподобной активности тотального пула протеасом и пулов 26S- и 20S-протеасом в ткани светлоклеточного рака почки по сравнению с неизмененной тканью. Обнаружено повышенное содержание α1-α7-субъединиц протеасом в ткани рака почки по сравнению с неизмененной тканью, что сочетается со снижением содержания LMP2- и РА28ß-субъединиц. В опухоли мочевого пузыря наблюдается увеличение активности 26S-протеасом, снижение экспрессии α1-α7 и повышение содержания LMP2-субъединицы в составе протеасом. Установлена связь активности 26S-протеасом нормальной ткани почки с отдаленным метастазированием. В ткани рака мочевого пузыря выявлены зависимость экспрессии α1-α7-субъединиц протеасом от размера опухоли, а также зависимость активности 26S-протеасом в опухоли от степени ее гистологической дифференцировки.

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