Local cytokine concentration in patients with non-muscle invasive bladder cancer of low malignant potential and with varying rates of recurrence

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Abstract

Aim. To investigate local concentrations and distribution of cytokines in tumor tissue and perifocal zone in non-muscle invasive bladder cancer of low malignant potential in patients with low and high probability of disease recurrence.

 Materials and methods. We have studied tumor and perifocal zone fragments of 31 patients with verified non-muscle invasive bladder cancer of low malignant potential and with different probabilities of recurrence. Fifteen (15) patients developed recurrences 6–9 months after combination treatment. The fragments of primary and recurrent tumors were  echanically disaggregated and centrifuged at 1500 rpm for 10 minutes. Levels of cytokines interleukin (IL) -1β, -6, -8, -10, -18, tumor necrosis factor α (TNF-α), interferon-γ (Vektor-Best, Russia), and epithelial neutrophil activating peptide 78 (ENA-78) (CXCL-5 chemokine) (Cloud-Clone Corp., USA) were measured in the samples by ELISA. Results were statistically processed using Statistica 13 software (StatSoft Inc., USA), and presented as median and interquartile range – 25th and 75th percentile (Ме [LQ; UQ]).

Results. Comparison of cytokine concentrations within the groups showed that the levels of inflammatory cytokines (TNF-α, IL-1β, IL-8, IL-6, IL-18) in tumor tissues were higher than in the perifocal zone tissues. This pattern was expected because tumor is the main site of inflammation. Comparison of these indicators between groups showed that in tumor tissues with an unfavorable course of the disease, namely disease recurrence, the levels of almost all inflammatory cytokines (TNF-α, IL-1β, IL-8, IL-6) were higher. A similar pattern was observed when comparing the levels of cytokines in the tissues of the perifocal zone. These differences were statistically significant. ENA-78 concentration was not determined in all cases.

Conclusion. The data obtained during the study indicates that in patients with unfavorable disease course (recurrence), tumor growth is associated with high expression of proinflammatory cytokines, which can subsequently lead to development of disease recurrence.

About the authors

L. I Belyakova

National Medical Research Center of Oncology, Ministry of Health of Russia

Author for correspondence.
Email: drlbelyakova@yandex.ru
ORCID iD: 0000-0001-7955-3473
SPIN-code: 3382-8559

Lyubov’ Igorevna Belyakova 

63 14th Liniya, Rostov-on-Don 344037

 

Russian Federation

A. N. Shevchenko

National Medical Research Center of Oncology, Ministry of Health of Russia

ORCID iD: 0000-0002-9468-134X

63 14th Liniya, Rostov-on-Don 344037

Russian Federation

O. G. Shulgina

National Medical Research Center of Oncology, Ministry of Health of Russia

ORCID iD: 0000-0001-6828-145X

63 14th Liniya, Rostov-on-Don 344037

Russian Federation

A. B. Sagakyant

National Medical Research Center of Oncology, Ministry of Health of Russia

ORCID iD: 0000-0003-0874-5261

63 14th Liniya, Rostov-on-Don 344037

Russian Federation

D. V. Burtsev

Regional Consultative and Diagnostic Center

127 Pushkinskaya St., Rostov-on-Don 344000

Russian Federation

E. Yu. Zlatnik

National Medical Research Center of Oncology, Ministry of Health of Russia

ORCID iD: 0000-0002-1410-122X

63 14th Liniya, Rostov-on-Don 344037

Russian Federation

I. A. Novikova

National Medical Research Center of Oncology, Ministry of Health of Russia

ORCID iD: 0000-0002-6496-9641

63 14th Liniya, Rostov-on-Don 344037

Russian Federation

E. V. Filatova

National Medical Research Center of Oncology, Ministry of Health of Russia

ORCID iD: 0000-0002-7904-4414

63 14th Liniya, Rostov-on-Don 344037

Russian Federation

V. K. Khvan

National Medical Research Center of Oncology, Ministry of Health of Russia

ORCID iD: 0000-0002-0036-7190

63 14th Liniya, Rostov-on-Don 344037

Russian Federation

I. A. Khomutenko

National Medical Research Center of Oncology, Ministry of Health of Russia

ORCID iD: 0000-0003-0003-8044

63 14th Liniya, Rostov-on-Don 344037

Russian Federation

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